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lung homogenates by elisa  (R&D Systems)


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    R&D Systems lung homogenates by elisa
    (A) Left: WT mice were administered PBS, CpG or Pm3 intrapharyngeally (i.ph.). Lungs were collected at seven days post treatment and homogenates were assayed for <t>mouse</t> <t>ACE2</t> by <t>ELISA.</t> Right: K18-hACE2 Tg mice were administered PBS, CpG or Pm3 i.ph., lungs were collected at 10 days post-treatment and homogenates were assayed for human ACE2 by ELISA, n= 3 – 8, data combined from 1 – 2 independent experiments. (B) Trem2 −/− or (C) Ccr2 −/− mice were given either PBS or 10μg CpG i.ph. seven days prior to being i.n. infected with 3.5×10 4 TCID 50 SCV2 (B.1.351), mice were euthanized 3 days later. Viral loads in lung are shown as measured by TCID 50 on Vero E6 cells, n= 3 – 8, data combined from 1 – 2 independent experiments. Geometric mean, significance determined by two-tailed Mann Whitney test, LD= limit of detection, n.s.= not significant.
    Lung Homogenates By Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lung homogenates by elisa/product/R&D Systems
    Average 92 stars, based on 3 article reviews
    lung homogenates by elisa - by Bioz Stars, 2026-02
    92/100 stars

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    1) Product Images from "The inflammatory microenvironment of the lung at the time of infection governs innate control of SARS-CoV-2 replication"

    Article Title: The inflammatory microenvironment of the lung at the time of infection governs innate control of SARS-CoV-2 replication

    Journal: bioRxiv

    doi: 10.1101/2024.03.27.586885

    (A) Left: WT mice were administered PBS, CpG or Pm3 intrapharyngeally (i.ph.). Lungs were collected at seven days post treatment and homogenates were assayed for mouse ACE2 by ELISA. Right: K18-hACE2 Tg mice were administered PBS, CpG or Pm3 i.ph., lungs were collected at 10 days post-treatment and homogenates were assayed for human ACE2 by ELISA, n= 3 – 8, data combined from 1 – 2 independent experiments. (B) Trem2 −/− or (C) Ccr2 −/− mice were given either PBS or 10μg CpG i.ph. seven days prior to being i.n. infected with 3.5×10 4 TCID 50 SCV2 (B.1.351), mice were euthanized 3 days later. Viral loads in lung are shown as measured by TCID 50 on Vero E6 cells, n= 3 – 8, data combined from 1 – 2 independent experiments. Geometric mean, significance determined by two-tailed Mann Whitney test, LD= limit of detection, n.s.= not significant.
    Figure Legend Snippet: (A) Left: WT mice were administered PBS, CpG or Pm3 intrapharyngeally (i.ph.). Lungs were collected at seven days post treatment and homogenates were assayed for mouse ACE2 by ELISA. Right: K18-hACE2 Tg mice were administered PBS, CpG or Pm3 i.ph., lungs were collected at 10 days post-treatment and homogenates were assayed for human ACE2 by ELISA, n= 3 – 8, data combined from 1 – 2 independent experiments. (B) Trem2 −/− or (C) Ccr2 −/− mice were given either PBS or 10μg CpG i.ph. seven days prior to being i.n. infected with 3.5×10 4 TCID 50 SCV2 (B.1.351), mice were euthanized 3 days later. Viral loads in lung are shown as measured by TCID 50 on Vero E6 cells, n= 3 – 8, data combined from 1 – 2 independent experiments. Geometric mean, significance determined by two-tailed Mann Whitney test, LD= limit of detection, n.s.= not significant.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Infection, Two Tailed Test, MANN-WHITNEY



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    (A) Left: WT mice were administered PBS, CpG or Pm3 intrapharyngeally (i.ph.). Lungs were collected at seven days post treatment and homogenates were assayed for <t>mouse</t> <t>ACE2</t> by <t>ELISA.</t> Right: K18-hACE2 Tg mice were administered PBS, CpG or Pm3 i.ph., lungs were collected at 10 days post-treatment and homogenates were assayed for human ACE2 by ELISA, n= 3 – 8, data combined from 1 – 2 independent experiments. (B) Trem2 −/− or (C) Ccr2 −/− mice were given either PBS or 10μg CpG i.ph. seven days prior to being i.n. infected with 3.5×10 4 TCID 50 SCV2 (B.1.351), mice were euthanized 3 days later. Viral loads in lung are shown as measured by TCID 50 on Vero E6 cells, n= 3 – 8, data combined from 1 – 2 independent experiments. Geometric mean, significance determined by two-tailed Mann Whitney test, LD= limit of detection, n.s.= not significant.
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    (A) Left: WT mice were administered PBS, CpG or Pm3 intrapharyngeally (i.ph.). Lungs were collected at seven days post treatment and homogenates were assayed for <t>mouse</t> <t>ACE2</t> by <t>ELISA.</t> Right: K18-hACE2 Tg mice were administered PBS, CpG or Pm3 i.ph., lungs were collected at 10 days post-treatment and homogenates were assayed for human ACE2 by ELISA, n= 3 – 8, data combined from 1 – 2 independent experiments. (B) Trem2 −/− or (C) Ccr2 −/− mice were given either PBS or 10μg CpG i.ph. seven days prior to being i.n. infected with 3.5×10 4 TCID 50 SCV2 (B.1.351), mice were euthanized 3 days later. Viral loads in lung are shown as measured by TCID 50 on Vero E6 cells, n= 3 – 8, data combined from 1 – 2 independent experiments. Geometric mean, significance determined by two-tailed Mann Whitney test, LD= limit of detection, n.s.= not significant.
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    (A) Left: WT mice were administered PBS, CpG or Pm3 intrapharyngeally (i.ph.). Lungs were collected at seven days post treatment and homogenates were assayed for <t>mouse</t> <t>ACE2</t> by <t>ELISA.</t> Right: K18-hACE2 Tg mice were administered PBS, CpG or Pm3 i.ph., lungs were collected at 10 days post-treatment and homogenates were assayed for human ACE2 by ELISA, n= 3 – 8, data combined from 1 – 2 independent experiments. (B) Trem2 −/− or (C) Ccr2 −/− mice were given either PBS or 10μg CpG i.ph. seven days prior to being i.n. infected with 3.5×10 4 TCID 50 SCV2 (B.1.351), mice were euthanized 3 days later. Viral loads in lung are shown as measured by TCID 50 on Vero E6 cells, n= 3 – 8, data combined from 1 – 2 independent experiments. Geometric mean, significance determined by two-tailed Mann Whitney test, LD= limit of detection, n.s.= not significant.
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    (A) Left: WT mice were administered PBS, CpG or Pm3 intrapharyngeally (i.ph.). Lungs were collected at seven days post treatment and homogenates were assayed for <t>mouse</t> <t>ACE2</t> by <t>ELISA.</t> Right: K18-hACE2 Tg mice were administered PBS, CpG or Pm3 i.ph., lungs were collected at 10 days post-treatment and homogenates were assayed for human ACE2 by ELISA, n= 3 – 8, data combined from 1 – 2 independent experiments. (B) Trem2 −/− or (C) Ccr2 −/− mice were given either PBS or 10μg CpG i.ph. seven days prior to being i.n. infected with 3.5×10 4 TCID 50 SCV2 (B.1.351), mice were euthanized 3 days later. Viral loads in lung are shown as measured by TCID 50 on Vero E6 cells, n= 3 – 8, data combined from 1 – 2 independent experiments. Geometric mean, significance determined by two-tailed Mann Whitney test, LD= limit of detection, n.s.= not significant.
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    (A) Left: WT mice were administered PBS, CpG or Pm3 intrapharyngeally (i.ph.). Lungs were collected at seven days post treatment and homogenates were assayed for <t>mouse</t> <t>ACE2</t> by <t>ELISA.</t> Right: K18-hACE2 Tg mice were administered PBS, CpG or Pm3 i.ph., lungs were collected at 10 days post-treatment and homogenates were assayed for human ACE2 by ELISA, n= 3 – 8, data combined from 1 – 2 independent experiments. (B) Trem2 −/− or (C) Ccr2 −/− mice were given either PBS or 10μg CpG i.ph. seven days prior to being i.n. infected with 3.5×10 4 TCID 50 SCV2 (B.1.351), mice were euthanized 3 days later. Viral loads in lung are shown as measured by TCID 50 on Vero E6 cells, n= 3 – 8, data combined from 1 – 2 independent experiments. Geometric mean, significance determined by two-tailed Mann Whitney test, LD= limit of detection, n.s.= not significant.
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    Image Search Results


    (A) Left: WT mice were administered PBS, CpG or Pm3 intrapharyngeally (i.ph.). Lungs were collected at seven days post treatment and homogenates were assayed for mouse ACE2 by ELISA. Right: K18-hACE2 Tg mice were administered PBS, CpG or Pm3 i.ph., lungs were collected at 10 days post-treatment and homogenates were assayed for human ACE2 by ELISA, n= 3 – 8, data combined from 1 – 2 independent experiments. (B) Trem2 −/− or (C) Ccr2 −/− mice were given either PBS or 10μg CpG i.ph. seven days prior to being i.n. infected with 3.5×10 4 TCID 50 SCV2 (B.1.351), mice were euthanized 3 days later. Viral loads in lung are shown as measured by TCID 50 on Vero E6 cells, n= 3 – 8, data combined from 1 – 2 independent experiments. Geometric mean, significance determined by two-tailed Mann Whitney test, LD= limit of detection, n.s.= not significant.

    Journal: bioRxiv

    Article Title: The inflammatory microenvironment of the lung at the time of infection governs innate control of SARS-CoV-2 replication

    doi: 10.1101/2024.03.27.586885

    Figure Lengend Snippet: (A) Left: WT mice were administered PBS, CpG or Pm3 intrapharyngeally (i.ph.). Lungs were collected at seven days post treatment and homogenates were assayed for mouse ACE2 by ELISA. Right: K18-hACE2 Tg mice were administered PBS, CpG or Pm3 i.ph., lungs were collected at 10 days post-treatment and homogenates were assayed for human ACE2 by ELISA, n= 3 – 8, data combined from 1 – 2 independent experiments. (B) Trem2 −/− or (C) Ccr2 −/− mice were given either PBS or 10μg CpG i.ph. seven days prior to being i.n. infected with 3.5×10 4 TCID 50 SCV2 (B.1.351), mice were euthanized 3 days later. Viral loads in lung are shown as measured by TCID 50 on Vero E6 cells, n= 3 – 8, data combined from 1 – 2 independent experiments. Geometric mean, significance determined by two-tailed Mann Whitney test, LD= limit of detection, n.s.= not significant.

    Article Snippet: Mouse and human ACE2 protein levels were quantified from lung homogenates by ELISA (R&D Systems #DY3437, #DY933).

    Techniques: Enzyme-linked Immunosorbent Assay, Infection, Two Tailed Test, MANN-WHITNEY